**************************************************************************************************************************************************************************************************** MOTIFSIM - Motif Similarity Detection Tool Version 2.2 **************************************************************************************************************************************************************************************************** INPUT **************************************************************************************************************************************************************************************************** Input Parameters Number of files: 5 Number of top significant motifs: 5 Number of best matches: 5 Similarity cutoff: >= 0.75 Matching motif database: Jaspar Urochordates Motif tree: Yes Combined similar motifs: Yes Output file type: All Output file format: All Input files and motif counts File name Count of motifs Dataset # DREME_DM230.txt 1 1 MEME_DM230.txt 20 2 PScanChIP_DM230.txt 14 3 RSAT_peak-motifs_DM230.txt 10 4 W-ChIPMotifs_DM230.txt 11 5 **************************************************************************************************************************************************************************************************** RESULTS **************************************************************************************************************************************************************************************************** ********************************************************************** Best Matches in Database for Each Motif (Highest to Lowest) ***************************************************************** Dataset #: 1 Motif ID: 1 Motif name: Motif 1 Original motif 0.000000 0.000000 1.000000 0.000000 0.000000 0.000000 1.000000 0.000000 0.000000 1.000000 0.000000 0.000000 0.000000 0.000000 1.000000 0.000000 0.000000 0.000000 1.000000 0.000000 0.000000 0.000000 1.000000 0.000000 0.148148 0.000000 0.851852 0.000000 0.000000 1.000000 0.000000 0.000000 Consensus sequence: GGCGGGGC Reserve complement motif 0.000000 0.000000 1.000000 0.000000 0.148148 0.851852 0.000000 0.000000 0.000000 1.000000 0.000000 0.000000 0.000000 1.000000 0.000000 0.000000 0.000000 1.000000 0.000000 0.000000 0.000000 0.000000 1.000000 0.000000 0.000000 1.000000 0.000000 0.000000 0.000000 1.000000 0.000000 0.000000 Consensus sequence: GCCCCGCC ************************************************************************ Best Matches for Motif ID 1 (Highest to Lowest) ************************************************************************ Motif ID Motif name Matching format of first motif Matching format of second motif Direction Position # # of overlap Similarity score MA0118.1 Macho-1 Reverse Complement Reverse Complement Forward 1 8 0.015649 Taxon: Urochordates Original motif 0.119048 0.357143 0.142857 0.380952 0.214286 0.047619 0.547619 0.190476 0.000000 0.000000 1.000000 0.000000 0.000000 0.000000 1.000000 0.000000 0.071429 0.095238 0.547619 0.285714 0.000000 0.000000 1.000000 0.000000 0.119048 0.166667 0.357143 0.357143 0.119048 0.333333 0.095238 0.452381 0.309524 0.333333 0.238095 0.119048 Consensus sequence: BGGGKGBYV Reverse complement motif 0.309524 0.238095 0.333333 0.119048 0.452381 0.333333 0.095238 0.119048 0.119048 0.357143 0.166667 0.357143 0.000000 1.000000 0.000000 0.000000 0.071429 0.547619 0.095238 0.285714 0.000000 1.000000 0.000000 0.000000 0.000000 1.000000 0.000000 0.000000 0.214286 0.547619 0.047619 0.190476 0.380952 0.357143 0.142857 0.119048 Consensus sequence: VMBCYCCCV Alignment: VMBCYCCCV GCCCCGCC- ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- Dataset #: 3 Motif ID: 24 Motif name: SP1 Original motif 0.000000 0.914286 0.028571 0.057143 0.000000 0.857143 0.028571 0.114286 0.000000 1.000000 0.000000 0.000000 0.114286 0.771429 0.000000 0.114286 0.057143 0.142857 0.428571 0.371429 0.000000 0.800000 0.028571 0.171429 0.028571 0.885714 0.000000 0.085714 0.000000 0.685714 0.085714 0.228571 0.171429 0.714286 0.000000 0.114286 0.085714 0.742857 0.085714 0.085714 Consensus sequence: CCCCKCCCCC Reserve complement motif 0.085714 0.085714 0.742857 0.085714 0.171429 0.000000 0.714286 0.114286 0.000000 0.085714 0.685714 0.228571 0.028571 0.000000 0.885714 0.085714 0.000000 0.028571 0.800000 0.171429 0.057143 0.428571 0.142857 0.371429 0.114286 0.000000 0.771429 0.114286 0.000000 0.000000 1.000000 0.000000 0.000000 0.028571 0.857143 0.114286 0.000000 0.028571 0.914286 0.057143 Consensus sequence: GGGGGYGGGG ************************************************************************ Best Matches for Motif ID 24 (Highest to Lowest) ************************************************************************ Motif ID Motif name Matching format of first motif Matching format of second motif Direction Position # # of overlap Similarity score MA0118.1 Macho-1 Original Motif Reverse Complement Forward 1 9 0.053545 Taxon: Urochordates Original motif 0.119048 0.357143 0.142857 0.380952 0.214286 0.047619 0.547619 0.190476 0.000000 0.000000 1.000000 0.000000 0.000000 0.000000 1.000000 0.000000 0.071429 0.095238 0.547619 0.285714 0.000000 0.000000 1.000000 0.000000 0.119048 0.166667 0.357143 0.357143 0.119048 0.333333 0.095238 0.452381 0.309524 0.333333 0.238095 0.119048 Consensus sequence: BGGGKGBYV Reverse complement motif 0.309524 0.238095 0.333333 0.119048 0.452381 0.333333 0.095238 0.119048 0.119048 0.357143 0.166667 0.357143 0.000000 1.000000 0.000000 0.000000 0.071429 0.547619 0.095238 0.285714 0.000000 1.000000 0.000000 0.000000 0.000000 1.000000 0.000000 0.000000 0.214286 0.547619 0.047619 0.190476 0.380952 0.357143 0.142857 0.119048 Consensus sequence: VMBCYCCCV Alignment: VMBCYCCCV- CCCCKCCCCC ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- Dataset #: 3 Motif ID: 27 Motif name: Klf4 Original motif 0.338561 0.018681 0.235701 0.407057 0.020276 0.002074 0.976267 0.001382 0.003223 0.002993 0.990792 0.002993 0.003221 0.008282 0.984817 0.003681 0.063693 0.441941 0.002529 0.491837 0.005064 0.003453 0.983656 0.007827 0.009671 0.018420 0.501727 0.470182 0.060872 0.010606 0.899700 0.028822 0.028400 0.030016 0.874856 0.066728 0.058742 0.660962 0.064755 0.215541 Consensus sequence: DGGGYGKGGC Reserve complement motif 0.058742 0.064755 0.660962 0.215541 0.028400 0.874856 0.030016 0.066728 0.060872 0.899700 0.010606 0.028822 0.009671 0.501727 0.018420 0.470182 0.005064 0.983656 0.003453 0.007827 0.491837 0.441941 0.002529 0.063693 0.003221 0.984817 0.008282 0.003681 0.003223 0.990792 0.002993 0.002993 0.020276 0.976267 0.002074 0.001382 0.407057 0.018681 0.235701 0.338561 Consensus sequence: GCCYCMCCCD ************************************************************************ Best Matches for Motif ID 27 (Highest to Lowest) ************************************************************************ Motif ID Motif name Matching format of first motif Matching format of second motif Direction Position # # of overlap Similarity score MA0118.1 Macho-1 Reverse Complement Reverse Complement Backward 1 9 0.000000 Taxon: Urochordates Original motif 0.119048 0.357143 0.142857 0.380952 0.214286 0.047619 0.547619 0.190476 0.000000 0.000000 1.000000 0.000000 0.000000 0.000000 1.000000 0.000000 0.071429 0.095238 0.547619 0.285714 0.000000 0.000000 1.000000 0.000000 0.119048 0.166667 0.357143 0.357143 0.119048 0.333333 0.095238 0.452381 0.309524 0.333333 0.238095 0.119048 Consensus sequence: BGGGKGBYV Reverse complement motif 0.309524 0.238095 0.333333 0.119048 0.452381 0.333333 0.095238 0.119048 0.119048 0.357143 0.166667 0.357143 0.000000 1.000000 0.000000 0.000000 0.071429 0.547619 0.095238 0.285714 0.000000 1.000000 0.000000 0.000000 0.000000 1.000000 0.000000 0.000000 0.214286 0.547619 0.047619 0.190476 0.380952 0.357143 0.142857 0.119048 Consensus sequence: VMBCYCCCV Alignment: -VMBCYCCCV GCCYCMCCCD ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- Results created by MOTIFSIM on 11-21-2023 10:01:18 Runtime: 65.835603 seconds MOTIFSIM is written by Ngoc Tam L. 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